<div><b>Background</b></div> <div>Acne vulgaris(AV) is the eighth most prevalent disease worldwide and affects pilosebaceous units with the multifactorial pathogensis. Antibiotics are considered first-line therapy in the management of moderate to severe acne. However,&nbsp;currently available agents may be associated with adverse effects (AEs), their prolonged usage potentially leading to antibiotic resistance ,which may occur in Cutibacterium acnes (C. acnes), and limit the efficacy of antibiotics for the treatment of AV. These limitations demonstrate a need for antibacterial agents for acne with improved safety profiles and a targeted, well-tolerated. An antimicrobial peptide(AMP) called GDP20 is novel , topical bactericidal against C. acnes and has an anti-inflammatory effect for the treatment of acne .</div> <div>&nbsp;</div> <div><b>Objectives</b></div> <div>To evaluate the anti-C.acnes activity of twice-daily GDP20 with help of MALDI NOF method.</div> <div>&nbsp;</div> <div>&nbsp;</div> <div><b>Material and method</b></div> <div>Twelve subjects with mild to moderate acne were enrolled into this pilot study. All subjects were under dermatologic care and were maintained on their acne agents throughout the study. Patients applied to the bacteriological analysis by method of matrix-assisted laser desorption/ionization Time Of Flight (MALDI-TOF) mass spectrometry at two weeks intervals. The results were documented at baseline and after visits and evaluated for clinical improvement and C. acnes growth.</div> <div>&nbsp;</div> <div><b>Results</b></div> <div>After 2 weeks of topical therapy with GDP20 in 41.7% (5/12) subjects having excellent results, 33.3% (4/12) with good results, 25% (3/12) with fair results. All subjects with mild to moderate severity of acne was marked by clinical remission. No serious AEs occurred. The levels of C.acnes contamination before and after GDP20 application are presented in Fig 1.</div> <div><img src="data:image/png;base64,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" alt="" /></div> <div>Fig 1. Comparison of the number of C.acne before and after treatment of Acne patients</div> <div>&nbsp;</div> <div>&nbsp;</div> <div><b>Conclusions</b></div> <p><span style="font-size: 10pt;">The application of topical agents GDP20 in this pilot study appeared to demonstrate a reduction in C.acnes growth and remarkable effect on acne manifestation. These results indicate the possibility to use GDP20 as a possible substitute for widespectrum antibiotics due to known bacterial antibiotic resistance.</span>&nbsp;</p>